The SPR slide is made of glass with a 50nm Au layer on top. Above this layer I can attach the streptavidin with one of the following procedures I found on another post for a bare glass slides:
1) Immersion of the glass substrate in a solution containing 5mg/ml of streptavidin in PBS or tris buffer at pH7. Then allow Streptavidin would adsorb on it. But the coverage is not perfectly uniform.
2) Immersing the glass for 20 min in 1Molar NaOH(aq), rinsing, then submerge the glass in a methanol solution of 3-aminepropyl-trimethoxy silane. Rinse with methanol. Rinse with water. Then submerse the amine modified glass in streptavidin solution.
However, I wanted to know how I can take this exact same SPR slide coated with streptavidin and remove the streptavidin coating so I can attach it with another fresh coating after use?
You can remove any organic layer from a gold surface by "Piranha solution".
https://en.wikipedia.org/wiki/Piranha_solution
However, you should consider that this solution is quite dangerous, potentially damages the slide, and may be difficult to dispose. In your case, it might be not worth the effort.
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