01 January 1970 0 7K Report

¿Cómo pueden los enfoques cualitativos en la investigación social ayudar a captar experiencias individuales que los estudios cuantitativos no logran reflejar, especialmente en el ámbito de la pobreza?

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PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

How to removal of dead cells and debris from organoid cultures?

I'm currently working with human pancreatic organoids and I am wondering to remove the dead cells and debris from health organoids, any suggestions would be appreciated!

17 July 2024 8,330 1 View

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View

Do anyone experiment occasional staining problems with cryoprotectant containing Polyvinyl-pyrrolidone and Ethylene glycole?

Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...

07 July 2024 8,200 2 View

Cp of Turbine is ever increasing with Tip Speed Ratio. What Could be the reason??

I am a student of Mechanical Engineering. I am doing CFD opf Archimedes Winf Turbine. I am trying to validate the result of Paper. But the problem is that tha value of Power Coefficient is ever...

05 July 2024 6,839 2 View

Dyslexia and Virtual Reality yes or no?

Do you think the use of virtual reality with dyslexia students is good?

23 June 2024 8,437 2 View

Can anyone recommend bibliography on the analysis of rage from an affect studies' perspective?

I'm analysing a novel in which the said emotion is fundamental to understand the development of characters. Thank you in advance.

22 June 2024 4,859 8 View

If I am doing a thematic analysis based on one group, is it okay to add insights from another group of interviewees?

I am doing a thematic analysis from interviewees done with my main group of interviewees. I also have done two interviews with another group of interviewees which provided interesting insights...

21 June 2024 3,305 6 View

Why our eyes can concisely definite left and right, high and low? But cannot near and far?

While analyzing the data of point cloud, i found some strange phenomenon about the precise. But I expect there is no best plan between LiDAR and binocular vision. However, why our eyes more...

21 June 2024 5,269 3 View

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