I have looked up on several lists of interferences and contaminants for ESI-MS but have not found what it could be. This big peak on m/z 265, negative mode won't leave.
Thanks so much for the insights. We are probably receiving some samples that are contaminated with detergents, so that might be the case. We have observed a little emulsion formation on some samples, but we thought that maybe some lipids could have provoked that.
The Possible Adducts are M +1, M-1, M+ Sodium , M-sodium, M + Acetate , M-Acetate which may be with 265 m/z. Adduct formation depends on inoization of compound in ESI APPI or APCI mode. It can be know during ionization.