DNA shearing by french press?

Is it possible to shear DNA by french press to get fragment size about 500 bp?

08 October 2017 1,014 12 View

Prediction tool for DNA-binding site?

Hi, could you recommended me any prediction software for DNA-binding sites of my protein? I have protein with predict structure using Psipred (pdb file) and fasta file with genome. I would like to...

26 July 2017 7,171 3 View

What experiences with Primocin do you have?

Hi everybody, I'm treating my contaminated cell cultures by primocin. What experiences do you have? What is the optimal length of treatment to obtain healthy cell culture? Do you use primocin all...

15 May 2017 1,440 2 View

Denaturing PAGE of DNA - how to get sharp bands?

Our research group has recetly bought CBS Scientific Adjustable Sequencing Kit (SG-400-20) and we are facing troubles with our electrophoretic separations because we are unable to obtain sharp...

26 February 2017 4,223 3 View

How to balance ChIP-seq library prep for multiplex sequencing?

I am preparing ChIP-seq libraries for Illumina multiplexing. I have large amount of the ChIP-input DNA compared to the ChIP-output. I wonder if it is better to keep the DNA amount for library...

16 August 2016 1,075 4 View

RNA immunoprecipitation - why Western blot doesn't work but RT-PCR does?

Hi everyone! I try to optimize immunoprecipitation method followed by RNA isolation and RT-PCR/real-time. I did it for a first time and I did not have any experience with this. I managed to...

02 August 2016 2,013 10 View

Has anyone experience with ChIP and bacterial transcriptional factors?

I am going to try this method for prokaryotic cells - gram negative bacteria. I want to define regulon of DNA-binding protein. My protein is non specific binding to dsDNA. Is it a problem?

07 February 2016 7,323 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

How to calculate ultraviolet dose in Gy or J/kg?

Currently I'm doing a literature review regarding the comparisons of different types of radiation such as uv, electron beam and gamma radiation. But I'm having a difficult time converting uv unit...

11 February 2021 7,642 3 View

Can I get the design of circular cylinder to fix thermocouple on the surface and cartridge heater in core ?

Respected Scientific Community. I'm interested to carry out experimental studies on a circular cylinder. But even after doing literature review I unable to get the exact design of the cylinder...

10 February 2021 6,221 1 View

How to provide reliability in P2P storage system?

I am familiar with a fact that in P2P storage distributed systems reliability is provided with redundancy using erasure code or simple file replication. I am interested if there are some other...

05 February 2021 1,325 3 View

I am struggling with the concept of preprint papers and wonder how people feel about them?

Specifically, how should we use them given they have not been peer-reviewed? Should we rely on the results reported in pre-prints for policy? I am really probing for thoughts on whether...

04 February 2021 3,175 15 View