I have read some papers regarding RNA extraction from recalcitrant plants. Most of them have used modified CTAB method. But i couldn't find the exact amount of the ingredients used. These are the materials used by most of authors:
* CTAB 2% (w/v)
* Tris-HCL 100mM (pH 8)
* EDTA 25mM (pH8)
* NaCl 2M
* PVP 2% (w/v)
* B-Mercaptoethanol (4%)
There are concentrations given, but not the amount that how much of these items should be mixed to make one buffer. Also what should be the storage conditions, and at what temperature should the buffer be added to the grounded tissue.
Experts please help
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