I have cloned my genes into the p18 and p25 vectors and transformed them into BH101 cells. And plated them onto MacConkey (deficient in lactose) agar plates supplemented with 1% maltose, I am leaving them to incubate for 48 hours. I am really confused though as to how my proteins are actually going to be induced in order to allow them to interact. I didn't add any IPTG and the proteins are under a lac promoter. Am I missing something?
Jess, acording to the Annex of the Euromedex manual (http://static.bioport.cn/data/upload/product/specification/396/1342594983673_396560.pdf) IPTG (final concentration 0.5 mM) is usually added to the medium in order to induce the full expression of the hybrid proteins. So, probably without the inducer only leaky expression will be achieved and you should add the IPTG to the plates.
I hope this might help you!
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