Inducible KO mice - FAQS.TIPS

18 December 2012 0 4K Report

I am looking for ER stress proteins inducible knockout in endothelial and/or smooth muscle cells or T cells

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What could be the possible reason that sintering of polycrystalline diamond fabricated with tape casting has suddenly started failing?

I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...

16 July 2024 3,212 5 View

pharmacogenomics and personalized medicine?

How is pharmacogenomics transforming personalized medicine, and what are the current challenges in its implementation?

05 July 2024 5,082 2 View

Anybody can assist me in Protein and magnetic beads seperation?

Hi, I hope you are doing well, I am working to remove DNA from protein sample by using silica hydroxode magnetic beads. I used different binding buffers but I din't get my protein band in initial...

02 July 2024 5,502 4 View

What may cause whitish discolor of polycrystalline diamond surface, and cracks near edge?

I am preparing polycrystalline diamond (PCD) with tape casting. The powder is 8~12 um. After drying of tapes, I place several tapes in Ta cup without warm-pressing. Next, debinding is carried...

23 June 2024 7,758 2 View

Need assistance in optimizing my protocol. Anyone can assist me about inducing hPSC or iPSC to MSC?

Dear Colleagues, I am currently working on a project that involves differentiating human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs) into mesenchymal stem cells (MSCs)....

19 May 2024 3,164 2 View

A Survey about Awareness and Trust In Self Driving Cars?

https://docs.google.com/forms/d/e/1FAIpQLSe4Ht6uSWs_IOArQZyHYtfYD7aEFJBxFnIgJ46yPQ_k7rbDjg/viewform?usp=sf_link

11 April 2024 3,417 0 View

How do mutations in microbial genomes lead to adaptations to different environments?

mutations play a critical role in driving the adaptation of microbial populations to diverse environments, allowing them to thrive in a wide range of ecological niches.

04 April 2024 8,855 3 View

What are the potential benefits and challenges of utilizing artificial intelligence and machine learning in drug discovery and development processes?

In my opinion, I could say: Benefits: Accelerated Drug Discovery Cost Reduction Optimized Clinical Trials Challenges: Dealing with big data Over-fitting and Generalization Human Expertise and...

01 April 2024 5,706 4 View

Proposing research ideas in the field of social psychology?

Hello to all possible researchers who have a developed research idea in the field of social psychology, specifically the behavior of individuals in groups

29 March 2024 3,809 6 View

Is it possibleto analyze a data set that has dependent variable time series and independent variables are cross sectional?

for example dependent variable is daily stock returns and independent variables are company characteristics such as firm size, leverage etc.

21 March 2024 362 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

checklust of the vaSCULAR FLORA NATIVE TO ITALY?

a second update to the checklust of the vaSCULAR FLORA NATIVE TO ITALY

24 July 2024 2,403 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

What is this animal ?

In saw this creature during a cable laying operation at 20km off the coast of Timor Leste on the timor sea at a depth of 1200 m.

14 July 2024 4,715 1 View