Induce anemia in rat using 2.4 dinitrophenylhydrazine ?

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Firas Jabbar

Dear Dr.Siham

Inject 20 mg\kg.b.wt.p.o once daily for 7 .

Thank you

Sihem Kariche

Thank you Dr. Firas the most problem for my protocol is the solubility of DNPH .For intramuscular injection is recommended to use as solvent a saline solution of 9 percent.

For my solution the DNPH is insoluble

It is a problem indeed , all the best

can i inject with dmso ther is no problem for rat

You can but some complication may see like Anemia & Peritoneal infection or some interactions this will be notice by your experiment.

so wich solvant i lust choos the is not details in protocols