In which medium to to prepare ABTS solution?

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Yurii V Geletii

The antioxidant assay must be performed in buffered solution, better in phosphate. ABTS stock solution can be prepared in water.

Omar Gonzalez-Ortega

The reaction in which ABTS participates is a redox reaction, thus it is affected by the pH if the reaction medium. As mentioned ABTS can be prepared in water but this is considering that its addition to the reaction medium will not affect the buffered pH. In my opinion, use buffer for everything.