Thank you Aaron!

hi Doris....

try this one....In vivo captured (IVC) cytokine assay (BD PharmingenTM). thats really easy....

Sounds interesting. I tried the Cytometric Bead Array for the detection of mouse cytokines, but the serum levels were too low. Do you think the IVC assay is more sensitive?

yeah... I think so.....

here's some information from the products...and I guess its effective than in vitro secretion assay or that bead array..

"Analysis of the types and levels of cytokines that are produced in vivo is often difficult. Many cytokines have short in vivo half-lives and do not accumulate to detectable levels as measured in serum samples. mRNA levels and in vitro cytokine protein secretion may not fully reflect cytokine protein secretion in vivo. The In Vivo Capture Assay for Cytokines, more directly measures the in vivo production of cytokine proteins. In this assay, mice are injected with 10 μg of a no azide/low endotoxin (NA/LE), biotin-labeled antibody that binds to the targeted cytokine protein as it is secreted, forming a long-lived soluble complex that accumulates in the blood. Mice are bled and the complex is captured from serum and added to ELISA plate microwells coated with antibody that binds to a different epitope of the same cytokine. The complex is detected by streptavidin-horseradish peroxidase followed by the addition of a chromogenic substrate solution.

fa aulia hi i want pcr test procdure please send [email protected]

I have done in vivo BFA, Longer should be fine - but it depends how much longer you are talking about ~8-12hrs I wouldnt imagine would be an issue but 48hrs I cant imagine it would work.