Which is the best optimization techniques to optimize parameters for heart disease data set?

Doing research in data mining. i have chosen classification and prediction. I have opted medical data sets like heart disease dataset from UCI machine learning repository. Completed imputation...

29 August 2020 7,746 5 View

Is this possible to dissect a larval genitalia?

Especially in the case of moths and butterflies...

05 August 2019 6,959 3 View

What EEC circuit is used in case of coating through impedance and for all coating this circuit will be same or not?

Electrochemical impedance : coated metal analysis which is immersed in NaCl solution.

18 July 2019 8,028 7 View

What solvent is best to deposit graphene oxide on steel through electrophoretic deposition cathodically?

graphene coating

20 April 2019 8,664 3 View

What can be the reasons for the control well also to turn blue (medium and inoculum only) with resazurin dye in MIC?

I am doing MIC with plant extracts against s. mutans. My control well which had the medium and the inoculum also remianed blue in colour after the addition of the resazurin dye and inclubated for...

16 March 2019 3,976 2 View

Tell me the position of three intense peak in XRD of graphene oxide?

XRS PEAKS OF GO

22 May 2018 204 10 View

Can you suggest me the best de -nova genome assembly tools ?

I have sequenced the complete genome of a plant species using two technologies The estimated genome size using flowcytometer is estimated to be 300Mb 1. Illumina(mate pair) 2. Oxford Nanopore (for...

16 December 2017 1,614 5 View

Where can I get the properties of Sapphire, to add as new material in Sentaurus TCAD?

In Sentaurus TCAD, we can define new material separately in the parameter file. However, where (standard reference) can I find all properties I need to define the Sapphire parameter file? I am...

27 April 2017 1,573 1 View

Invitation for MS contribution in edited book Plant Biotechnology

01 January 1970 5,818 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

No title

22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View