Hi everyone,

I am currently doing stereotaxic surgeries in mice and I am using the retrograde tracer CTB Alexa Fluor 488 Conjugate. I am performing injections in the mouse BLA of 300nl CTB488 in a 1:10 dilution but I don't really see as many projections in the PFC as I expected from literature. I return the mice in their home cage for 2 weeks before next experiment.

I was thinking that made it shouldn't be diluted or maybe it needs more time after surgery.

Your input would be really helpful.