I synthesized some (metallo) porphyrins. I am now characterizing them by Mass spec and also confirming their purity by Elemental analysis. The values I got are a little deviated from the calculated values. What is the allowed limit of deviation for mass spec and elemental analysis in porphyrin chemistry? I will appreciate some references too. Thank you.
Hello Sebastian,
Perhaps you could tell us the values you expected vs observed? What sort of concentration were you expecting to see? What type of mass spectrometer were you using; this will possibly influence how much variation is plausible (measurement error) and how much is experimental (preparation method, dilution factors, pipette error etc).
With respect to Mass spectra, the mass error should be within 5 ppm (for ESI-HRMS). You can use the following website to calculate or you can do the calculations on your own. Those are simple enough.
https://warwick.ac.uk/fac/sci/chemistry/research/barrow/barrowgroup/calculators/mass_errors Sebastian Oloo
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