In the case of a histoenzymology it is possible to realize it on paraffin sections or the use of freezing is obligatory?
Dear Rafik, Why you dont ask me this question at the laboratory.
Of course, you can use a paraffin sections to perform the immunoenzymatic reaction withs specific antibody against CYP 19 protein (p450aromatase).
If tissues are already frozen at -80°c, you need first
1. Perform thin sections on tissue Teck support. (3-5um) using cryotome
2- Slides must be imbeeded in prefixative PAF 1%
3. Second fixative step in PAF 4%
4. You can perform yhe inmunoreactive reaction specific first antibody, thn second antibody to stop reaction
You can also use a quantitative spectrophotometric method by an enzyme assay kit,, including several substrates and ingredients. The protocole guide allow you to perform the multiples following steps. In this case, frozen organs must be prealably crushed (broyés) in a homogenate solution, with a adequate dilution.
best regards,
Yacine Soltani
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