How to compute the distance in km between two points inside the Earth?

Hello everyone. I am looking for a simple formula to compute the distance in Km between two points inside the Earth. I do not mean the usual problem between to cities (distance along the surface)...

02 February 2021 3,455 4 View

How can I quantify background noise in a bioacoustic file?

I am using Audacity to analyse the presence of birds and frogs recorded with a SM4 recorder (Wildlife Acoustic). Background noise (rainfall, wind) is an important variable to consider that can...

28 January 2021 2,989 3 View

How can I project 3D objects on 2D images??

Hello! From 3D images stacks I have recovered some objects. Now I need to project those objects on 2D for further analysis. Any idea, any software, plugin, etc?

21 January 2020 4,144 4 View

Does anybody knows if there is a metodology of acute ethanol toxicity for neuroprotection studies that uses functional foods?

I recently read an article regarding the use of melatonin as a neuroprotective agent using acute ethanol exposure for 1 day and it had good results, I want to know if exists an article using...

03 January 2020 9,709 2 View

Is there a way to normalize my western blot bands since i loaded equal volume of seminal plasma (not based on protein concentration)?

I loaded 10ul of raw seminal plasma sample + 10 ul Bolt LDS buffer +4ul Bolt reducing reagent. I did the transfer using iBlot2 dry transfer system and used One Pierce ECL protein detection kit....

06 May 2019 6,912 3 View

Is there an acceptance criteria or limit for swelling index/behavior for sustained release matrix tablets?

I am doing a project on Matrix Tablets using natural products as binders (release retardant polymer) and I came across Swelling Behavior/Index. Is this necessary? I know swelling is vital for gel...

03 December 2017 8,272 2 View

I have two solution: Solution A and Solution B. I will mix the two. Which wavelength should I use for UV-Vis Spectroscopy?

I have two solution: Solution A and Solution B. I will mix the two. Which wavelength should I use for UV-Vis Spectroscopy? Solution A is pink while Solution B is green in color. If I will mix the...

16 October 2017 6,761 3 View

Why I have low reproducibility in my real time pcr?

Hello , I'm quantifying bacteria by real- time PCR . I use plasmids with the target sequence as standards. The reproducibility is not good, one time i have 95% of efficiency and the next day is...

18 July 2016 4,605 7 View

How I can know the degree of crosslinking of a polymer?

Is it known that there is a variation in the degree of crosslinking varying synthesis parameters, but that technique I can use to know this degree?

28 April 2015 3,119 6 View

Can anyone provide recipes to make artificial agar spheres with varying Brix degree levels taking into account sucrose, glucose and fructose?

How many analytic recipes do you know to perform a given level of Brix in an artificial fruit?

13 February 2015 7,756 2 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

Blast analysis of RC,UHPC slabs using material model *MAT_72R3 in LS-DYNA?

20 February 2021 5,984 3 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View

Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,090 3 View

Is nr blast important?

Hello, I have designed a specific primer for my Gene, when I blast this primer, it just finds the mRNA of the gene, but in nr blast, I have found some CDSs that are not relevant to my Gene. Do...

05 February 2021 5,842 3 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Abaqus - plate is going up under gravity+blast loads?

Hi, I have modeled concrete plate with reinforcement supported on 2 concrete block. between concrete block and the plate I have added regular contact. I have created 2 steps: 1) only gravity load...

30 January 2021 2,772 1 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View