Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

What is the difference between Q sepharose column and DEAE Sepharose column?

I am working on protein (lipase enzyme) purification. After using the Sephadex G-25 fine column, which column (Q or DEAE sepharose column) should I use? What are the buffers should I select? Thank you

16 February 2021 6,921 5 View

How to use ArcGIS to separate certain % of data points scattered over certain area?

I was able to make a grid using fishnet in ArcGIS. But after that, I need to select 20% of points from each cell and make a different file containing ids of the data points as testing data set....

07 January 2021 9,533 1 View

How to quantify the two components overlapping in HPLC?

I am analyzing the biodiesel using reverse-phase HPLC. Methyl Oleate and Methyl Palmitate are overlapping in a single peak. How can I quantify the individual components from the same peak?

14 November 2019 1,406 9 View

I am working on a medical imaging research and if anyone knows where I could find CT scans of the brain with and without contrast agents?

I have looked up Cancer Imaging Archive but I need a bigger dataset. Can anyone help me out with some good places to look at for more images with and without contrast agents?

28 October 2019 4,219 3 View

Formula to calculate the plating cell volume based on the doubling time, culturing time, desired confluency and the volume of the culture flask?

Hi I am working on various cancer cell lines now. For my experiments, doubling time is really very important. Because even a small percentage of dead cells will affect my measurements. I have...

09 June 2019 6,592 6 View

Difference in carbon footprint between imported and locally produced food?

Field to fork studies....any papers or on going research?

28 May 2019 5,334 3 View

How to troubleshoot the IF staining problems in skin tissues?

Hi We are working in the area of psoriatic diseases. We are everytime getting the problem in expression of protein in dermis. We unable to see the expression in epidermis. Even, we did IHC too...

29 November 2018 4,074 1 View

Why is in situ hybridisation preferred over immunohistochemistry in paraffin embedded mouse embryo sections?

I am trying to characterize early embryos and most literature I have read seems to prefer in situ hybridization over immunohistochemistry to study expression profiles of different proteins. Does...

13 November 2018 8,911 1 View

What is the Power-Train-Efficiency of Diesel engine locomotive in %?

I am thinking the efficiency to be around 15%. Please help me with the exact value.

25 October 2018 8,025 4 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

Which one is better for storing energy: Fuels synthesized from CO2 or Hydrogen?

Based on educated guesses, which one of the following promises a better future for storing energy for grid scale, automobiles etc as opposed to batteries: 1) Fuels synthesized form CO2 2)...

02 February 2021 8,741 6 View

I want to extract engine oil from a soil sample,please how much amount of anhydrous sodium sulphate am i going to add to 10kg soil?

Engine oil extraction from contaminated soil,10kg of contaminated soil+20ml of dicholoromethane+? of anhydrous sulphate

01 February 2021 9,509 1 View

How to build aero engine fuel system online prognostics simulation model using Matlab, Simulink and Simscape?

How to build aero engine fuel system online prognostics simulation model using Matlab, Simulink and Simscape?

17 January 2021 5,181 1 View

Which search engines can I use to get the source of a certain microbial strain?

I am searching for a certain website through which can I get the source of certain microbial strains, especially probiotic strains I mean a search engine contains a database of the identified...

20 December 2020 5,144 5 View

Why my deltaATG mRNA is in polysome fractions ?

Hello everyone, I'm doing polysome profiling to answer a specific question. I study a gene which produce an alternative transcript and it's translation is controversial (extremely lucky if you...

16 December 2020 7,622 2 View

Agarose gel electrophoresis: only ladder is showing up?

07 December 2020 4,808 3 View

Has anyone compared supervised classification methods using google earth engine?

Good Morning! Has anyone compared supervised classification methods using Google Earth Engine? Could you comment on what are the criteria you used to decide on any method?

29 November 2020 544 4 View

What will be a futuristic city look like?

The extent of usable land will decline due to rise in sea levels. Many islands will be disappeared. People might have to carry oxygen tanks to breathe. Technological development won't go any...

29 November 2020 6,276 4 View

I am looking for diesel generator protection system in a solar hybrid system?

For hybrid solar project I am looking for a diesel generator protection system.. The solar system runs normally connected to the grid and excess power is normally exported to the grid. My...

07 November 2020 9,642 5 View