small unit 13 wells, of Electrophoresis, I am using 80 volts for RAPD primer analysis, but now i am using 30 wells unit then what is voltage for same result.
Hi Mahesh,
For deciding the voltage for electrophoresis, the number of samples are not to be considered. The deciding factor is the distance between the electrodes of the unit. In addition the size range of the amplicons may also be taken into account.
I don't think changing voltage will effect the electrophoresis results. keep gel concentration same. Run for appropriate distance.
Voltage is run throughout the entire gel, resistance of the voltage does not change by using more DNA samples. Also you mentioned that you are using 30 wells, If you use a Bio-rad Tray, LabTIE provides gel combs which have 33 slots. = 2 x multichannel including marker slots!
http://www.labtie.com/gel-electrophoresis-combs
Just send them an e-mail
Good luck:)
When you use agarose gels having many wells (usually it is 8 or 10 wells in small units) as in this case 33 wells, I think you must be using a larger size electrophoresis tank. Hence it will be good if you can measure the distance between two electrodes. For RAPD usually 1 or 1.5% w/v gels are used, and 5volts per cm is the voltage required to run gel that results in quality resolution. If you find that distance between electrodes is 25cm then you should use 25 cm x 5 = 125 volts for running the gel.
Dear Yashwant Chavan ji,
Really i am struggling with this problem of small tank size and big size tank. so, now i got the answer from ur side. means that when i found result in small tank at 80 volt and i want to to these same result then need to multiply by 5 with increase size of between the electrodes.
Thank u so much u all spend ur valuable time for my query.
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