In cytotoxicity assay, could you suggest to me, instead of AgNPs solution why AgNO3 solution used in testing cytotoxicity and genotoxicity?. what is the role of AgNO3.
Really I couldn't catch you ask exactly but some of my recent papers attached, i hope they present some answers@ Azharuddin Daphedar
The natural frequency of the bridge is the eigenvalue of smallest magnitude of a system that models the bridge, can anyone tell me how it is work?
24 January 2022 8,515 5 View
Here I am requesting if have any book chapter's regarding Nanotechnology please suggest me I want to contribute it. Thanking you
10 November 2019 2,849 3 View
I am working on biogenic silver nanoparticles synthesized by different plant extracts....so further I want to continue to my work on mitotic chromosomes for that I want make it different...
03 April 2018 572 0 View
How many HeLa cells need to seed in 6 cm plate to get confluent in 24 hours?
10 May 2016 4,403 2 View
Marine core samples dated ranging in late Quaternary time frame (0-12Ka).
08 September 2015 5,663 3 View
I want to know the sea level at different times in my holocene sediment cores from NE Arabian sea. Is there any equation to convert P/B foraminiferal ratio to water depth directly?
11 August 2015 3,935 11 View
Dear all, Wonder if any one can through some light on estimation of corrected troponinT on a post dialysis sample if we have a haematocrit and ultrafiltration volume. I have Pre- and post-dialysis...
20 June 2015 8,487 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View