In a tropical condition, temperature is highly fluctuated and no extreme hot and extreme cold ? If we use bacteriological incubator to grow the bacteria (mesophiles) isolated from natural environment like soil, incubator provides a constant temperature (37C). Basically is it correct or not ? If a biotic organism growing in fluctuated environment, if it is exposed to an in vitro condition with constant temperature, it may affect the growth of bacteria ? But if a bacteria isolated from human instestine, then 37 C is a must, isn't it. If the above statement is Okey, then what is the need of bacteriological incubator are a basic instrument in microbiological lab especially in tropical countries like India or in Asian continents?