Hi every one
I need informations about the implication of inflamatory proteins in systemic sclérosis (Articles, books.. ) I really appreciate ur help
Hi, I have a Gaussian cube file (.cube) that I obtained with gmx spatial. It represents the spatial density of cholesterol during a simulation (Protein in a membrane with explicit solvent). I...
03 April 2019 9,209 1 View
Hi, I have a Coarse-grained simulation of a dimer with 5 transmembrane helices for each monomer and I would like to calculate the tilt angle of each monomer with the respect to the membrane...
31 December 2018 6,042 2 View
Dears, I run several Coarse grained simulations of a transmembrane protein using Martini FF. In order to do a deep analysis of my systems, I backmapped a part of the trajectory from CG to AA....
09 October 2018 4,416 2 View
Dears, I run MD simulation on a transmembrane protein. When I visualised the frames, it seemed that I have some loss in alpha-helix (TM region). How can I quantify this? NB: I used timeline of...
07 August 2018 4,220 4 View
Dear all, I would like to simulate a protein which is located in the Mitochondrial Outer Membrane (OMM). I was searching in the literature about lipid composition of this latter (in Mammals) but...
01 February 2018 5,091 2 View
Dear all, I'm running simulations on membrane proteins (POPC) using Desmond software and I would like to use GROMACS tools to analyze certain parameters related to the membrane. Exemple: -...
02 March 2017 1,072 2 View
Dear all, I run MD simulations using GROMACS of a protein in the apo and holo form and I have to analyse the differences between the two forms. What kind of tests that can be performed? Thank...
10 November 2016 8,182 5 View
Dear all, I'm working on simulating a homodimer with its cofactors (hem, THG) and its substrate with GROMACS. Since my system is complex, I decided to start first with a monomere taking only...
04 May 2016 706 2 View
Hello, I have missing atoms and residues in my PDB file and I need to fix them in order to proceed with pdb2gmx (GROMACS). I used swissModel but the generating file is lacking the final residue in...
04 May 2016 7,559 6 View
Hi every one, i need ur help please: i'm preparing my final project about the implication of autoantobodies and inflammatory proteins in systemic sclerosis and i can't find any articles about...
04 May 2011 8,324 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...
15 June 2024 3,208 4 View
define cd
06 June 2024 9,533 5 View
I'd like to receive notifications of call for papers for any journal pertaining to my expertise (immunology, rheumatology, immuno-mediated liver involvement) from researchgate. Thanks in advance...
01 June 2024 1,352 0 View
Hi all, I'm trying to validate an ELISA against anti-drug antibodies in which I use a immunized rabbit serum as positive control. Rabbit was a NZR, immunization protocol was "standard" but I...
13 May 2024 2,828 0 View
how can immunological interventions potentially address these issues?
04 April 2024 4,951 1 View
I am a PhD candidate in immunology at AWKUM with a research focus on chronic inflammation, particularly the role of macrophage cells. I am writing to express my keen interest in your research on...
28 March 2024 7,798 0 View
I have several pairs of parameters (obtained from females and males) and want to find the difference in correlation between the two sexes for each parameter, but also want to give a weight so that...
11 March 2024 1,000 4 View