Hi, I'm a neuroscience guy but unfortunately I'm now stepping into the B-cell system.
We have plenty of experience playing around with the basic biocheminstry assays, but recently I'm testing the Immunoprecipitation with a receptor expressed in B cell, and unexpectedly we are suffering from the endogenous heavy and light chain in B cells for the antibody-immunoprecipitation.
I have tried to do a pre-incubation with the cell lysate with the Protein A beads before the antibody-IP but still couldn't get rid of it.
Does anyone have some experience doing such kind of assays, or where could I find any applicable protocol dealing with this issue? Thanks!!
Fred
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