immunomodudulatory effects

More John O. Ogunbileje's questions See All

growth kinetic/cell viability study in toxicology study

Can anybody help me with detailed protocol in carrying out growth kinetic/viability study of toxicant in a 12-well plate.

01 February 2011 136 1 View

MACROPHAGES

Can any one tell me if there is any source of Human alveolar macrophages in the market, I dont want to use rat alveolar macrophages.

11 December 2010 8,277 0 View

immunomodudulatory effects

to examine the effects of immunotoxic effects of cement dust which will best, mouse or rat

04 May 2010 4,410 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Is it possible to extract DNA or RNA from paraffinized biopsies ?

My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.

06 December 2020 7,964 6 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

27 October 2020 2,068 2 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View

John O. Ogunbileje

NICE HAVING THIS GROUP

Suping Peng

Hey guys, I am going to stain CD4, CD8 and MHC II also in IHC, I was told that I should use non-fixed section for these. What kind of sample do you use Roberta?

Angela Evan

what is IHC?

Mebrahtu Tedla

There are a lot of commercial anti CD4 and anti CD8 markers and you can detect your cells using immuno histochemistry. Your question lacks clarity and i can't give better suggestions than this.apologies for that!