Immunology work - FAQS.TIPS

More Praveen Kumar Vemuri's questions See All

How can I construct a super mix DNA ladder?

I need to construct super mix DNA ladder from the DNA extracted from various bacterial species using restriction enzymes. I dont want to use PCR primers to amplify. Literature? Experience? New ideas?

02 March 2014 4,999 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those vacuolated structures in my tilapia intestinal cross-sections (H&E stained)? Do the sections exhibit signs of enteritis?

Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...

15 June 2024 3,208 4 View

Is there anything called cd 12 in immunology?

define cd

06 June 2024 9,533 5 View

How can I put an alert to receive notifications of call for papers in my research field?

I'd like to receive notifications of call for papers for any journal pertaining to my expertise (immunology, rheumatology, immuno-mediated liver involvement) from researchgate. Thanks in advance...

01 June 2024 1,352 0 View

How many percent of specific antibodies in serum after rabbit immunization ?

Hi all, I'm trying to validate an ELISA against anti-drug antibodies in which I use a immunized rabbit serum as positive control. Rabbit was a NZR, immunization protocol was "standard" but I...

13 May 2024 2,828 0 View

What role do autoimmune disorders play in causing infertility?

how can immunological interventions potentially address these issues?

04 April 2024 4,951 1 View

PhD candidate with a research focus on chronic inflammation particularly macrophage cells so i need professor information this area??

I am a PhD candidate in immunology at AWKUM with a research focus on chronic inflammation, particularly the role of macrophage cells. I am writing to express my keen interest in your research on...

28 March 2024 7,798 0 View

How can I add weight to difference in correlation between 2 groups to also reflect the higher correlation values in addition to the difference?

I have several pairs of parameters (obtained from females and males) and want to find the difference in correlation between the two sexes for each parameter, but also want to give a weight so that...

11 March 2024 1,000 4 View

Daniel Corcos

Theoretical work: http://iridia.ulb.ac.be/bersini/Self-NonSelf/

Praveen Kumar Vemuri

Thank you Daniel Corcos

Mebrahtu Tedla

It is like fish without water but immunology can be survived without animals if you need to work on immunoepidemiology which bridges the impact of individual immunity for microbials on disease dynamics in a large population.