From the reading I've done, it appears that the requirements are: 3 chambers, a small chamber at either end, isolated by 'grease' that the ends of the nerve go into, and a central chamber that holds most of the nerve. The poles of the bipolar stimulator go into the proximal and central chamber, while the recording electrode goes into the distal chamber, with the reference in the central chamber (and the nerve is crushed at the point where it travels through the grease gap entering the distal chamber. Am I missing anything, or is anything wrong? I want to use it to measure conduction velocity in the nerve.

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