VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Sb2Te3 thin film annealing and damage(break)?

21 January 2021 9,642 2 View

Can I calculate the average from the data which are collected every 30 minutes for ANOVA?

I artifically elevated the air temperature in the heated plot by 3-celsius degree compared to that in the control for 7 days. I want to figure out the effect of the temperature manipulation on...

21 January 2021 1,185 8 View

When comparing two very similar surveys taken over time, but with different sample size, what would be the best way to approach this problem?

Hi, I'm currently analysing a survey for a university course design, and some guidance would be very much appreciated. The students who are enrolled in a (year-long) course were given a link to...

08 December 2020 6,624 9 View

When analysing survey using Likert scale, is it okay to use Friedman's test?

Hi, I'm currently analysing a survey for a university course design, and some guidance would be very much appreciated. The students who are enrolled in a course were given a link to participate...

06 December 2020 6,060 2 View

What is wrong with my western blot transfer system? (smearing is occured)?

I'm now doing western blotting for several proteins. but transfer is not perfectly happened. bands are looks like smeared or smudged. And it seems to the lower MW bands are more affected. what did...

06 December 2020 9,153 5 View

Which time-serie model is good when using concatenate data?

I would like to make a model using sequence datasets. As I've searched that , many LSTM, RNN tutorials use long-sequential data such as weather in 1950-2020. However, my datasets are short...

24 November 2020 808 3 View

Transformation of Bacillus subtilis?

Colonies appeared after transformation of pHT01 plasmid into Bacillus subtilis, and band was confirmed by performing colony PCR. However, if the colony is picked and grown (in LB+antibiotics) and...

27 October 2020 609 4 View

Could you answer some basic questions on CRISPR-cas9?

Hi, I would like to ask simple some questions related to CRISPR-Cas9 that I could not understand by reading some basic articles on it. I am not in that scientific field so my questions might be...

21 October 2020 2,439 2 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View