Why might some bands on an agarose gel appear above the top of the ladder?

In our experiment, the top of our ladder indicates 12,000bp, and in subsequent lanes we have samples where the band shows up slightly above the top of the ladder (however we know for a fact that...

27 January 2021 5,769 3 View

How to thaw whole blood for ELISA?

Hi all, I have frozen (-20C) samples of whole blood from mice from a recent experiment. I need to find a way to thaw them so as to extract sera for an ELISA, while minimizing hemolysis and...

08 December 2020 1,004 3 View

Must I remove Triton X-100 and/or NaCl before before Bradford assay and/or SDS-PAGE (with BME)?

Hello! I'm purifying a protein and have several questions about what is appropriate to include in the extract solvent at different stages of the purification and characterization phases. (1)...

05 August 2020 4,625 7 View

Can lead toxicity cause diabetes?

Can high exposure to lead oxidise pancreas beta cells, reducing insulin secretion? If so, what type of diabetes would this be?

30 June 2020 2,455 2 View

Please, recommendations needed for appropriate test instruments for self-perception and self-efficacy relevant to the workplace?

I am interested in the influence of skill bias perception on employees' self-perception and self-efficacy in the workplace. Looking for test instruments that measure attitude, behavior, and...

07 February 2020 5,964 3 View

At a loss. PCR that was working fine suddenly producing smears. Other reactions work perfectly, have switched all reagents. What to try next?

I'm trying to linearize a non-commercial vector for some cloning. We have primers that back up to one another, so when it amplifies outwards it opens up the plasmid. Or, at least, it did. Without...

07 February 2019 6,741 13 View

How can one prepare activated carbon from a specific carbon material for adsorption and desorption of a specific cation like Na+ or K+ or Ca2+?

The cation source will be an industrial and agricultural waste.

25 January 2019 6,084 1 View

Is there an appropriate method for the application of extreme value statistics to the analysis of water quality data?

The use of GEV and GPD has received much attention in hydrology for use in extreme precipitation and flood analysis. Methods of parameter estimates such as Maximum Likelihood Estimates and Linear...

25 March 2018 4,980 8 View

Matlab Cellular Automata help? For some reason I am not getting the correct Image?

function Life1D(ruleNum, key) key1 = key; ruleNum1 = ruleNum; rule = toBinary(ruleNum); initialRow = firstRow(ruleNum, key); disp(['Life1D ' num2str(2*key + 1) ' ' num2str(key +...

30 January 2018 9,992 3 View

Special precautions for immunohistochemistry co-stain with three different methods?

I'm staining a paraffin-embedded bone marrow section using three different methods: Traditional": (primary RAT Ab, secondary MOUSE ab conjugated to HRP),

18 September 2016 2,560 1 View

Can someone help me with this error that I am getting while estimating a VAR model in R?

I am using annual time series data to estimate a VAR model in R. However, when I run the command to find the optimal lag length, I get the following error: Fehler in VARselect(data, lag.max = 10,...

18 February 2021 9,647 3 View

Can I use breaking series as a regressor in a VAR model?

Context: I have two series, price and sales. Sales is mean-reverting stationery but price is stationary only after controlling for an intercept break. I want to set up a 2-equation VAR model...

14 January 2021 6,824 12 View

How to handle missing values?

Dear colleagues, What can you suggest in order to fill the missing values, as you can see in the picture? Best Ibrahim

24 December 2020 4,834 5 View

How can i get the data for the FTSE/JSE Responsible investment top 30 index (J110)?

Hi All , Please can you recommend a free database (i already use yahoo finance but it's not avaible) ,or if someone has already worked on this index for the period of 2015 it would be great to...

22 December 2020 247 3 View

Where can I find data about quantitative and qualitative techniques being used by different venture capitalists say Accel, Benchmark etc?

I want to write thesis by making comparison of techniques being utilized by VCs to predict risk and make investment decisions. I think this comparison will help to add value by revealing new...

04 December 2020 6,680 4 View

ARDL bounds test on Eviews: does multicollinearity impede the model from running?

Hi everyone, I hope I can get some help. My group is running an ARDL bounds model to test the Environmental Kuznets Curve in Cyprus. We're trying to perform it on Eviews, but we can't seem to be...

02 December 2020 9,230 4 View

Difference between ECM Regression and CEC Regression?

What's the difference between the ECM Regression in the ARDL Error Correction Regression and the Conditional Error Correction Regression in the ARDL long run form and bound test as seen in EViews

02 December 2020 6,770 1 View

How to conduct CUSUM and CUSUMsq test after the inclusion of an exogenous dummy variable in my ARDL model?

Hi all, I have been struggling a great deal with this matter and as such I hope you could provide me with answer. I have added a dummy variable to my ARDL model to improve its stability (Eviews)....

01 December 2020 1,071 5 View

How should I interpret my results if my VAR estimates are different then my IRFs?

Dear community, For my thesis, I am busy finding possible relationships between bitcoin returns, several index funds returns and macro economic variables. After running a Dickey-Fuller test on...

30 November 2020 4,923 3 View

How to run an MGARCH-BEKK MODEL in eviews and interpret the results?

19 November 2020 4,350 3 View