Can anybody recommend good antibodies for detecting the NMDA-receptor subunits NR1, NR2a and NR2b in PFA-fixed frozen mouse brain ? Thanks
We can not vouch for quality on specific Abs, but we can offer a good list of sources as a starting point for you. Just use our antibody search engine(http://www.linscottsdirectory.com/search/antibodies) and search for NR2a etc. You can select Mouse from Reactive Species pull down, and IHC from Applications. You should get a pretty good list of suppliers. Good luck.
We use antibodies from Chemicon in our lab. They work well on our vibratome sections. But I don't know if they work on frozen material.
Hi Peter, if you don't have any luck, please consider custom aptamer development. We can make aptamers for you rather quickly and cheaply. www.BasePairBio.com
i have used antibodies from BD biosciences as well as polyclonal from sigma and they have worked in both free floating and frozen sections
The only NR1 antibody I've ever used that only gave one band on a western blot was from Epitomics. I've used it on formalin-fixed primary neuronal cell cultures and it worked well too. It was E16 rat, DIV14 or so though, not mouse tissue.
check out the following site and compare for yourself
http://www.labome.com/gene/human/GRIN2A-antibody.html
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