It is important to asses and compare performance of these instruments by running a suitable standard/s under identical method settings. For an experienced mass spectrometrist, comparing instrument specific settings can be informative as well. In general, for any two mass spectrometers of the same make and model measurement outcomes will depend on individual instrument tuning, cleanliness, and age.If one is tuned for small molecule analysis, while another is a dedicated proteomics platform, their best performance may fall into different mass ranges. Best wishes.

It is important to asses and compare performance of these instruments by running a suitable standard/s under identical method settings. For an experienced mass spectrometrist, comparing instrument specific settings can be informative as well. In general, for any two mass spectrometers of the same make and model measurement outcomes will depend on individual instrument tuning, cleanliness, and age.If one is tuned for small molecule analysis, while another is a dedicated proteomics platform, their best performance may fall into different mass ranges. Best wishes.

The instrumental parameters depend on the user. If you have two instruments of the same make and model, you should keep the parameters (including the ion-source ones) as close and as constant as possible, and even then you're not absolutely guaranteed to obtain exactly the same result. After all, even the ambient temperature can shift your mass scale. A suitable internal mass standard would be very helpful.

Hi Oliwia Bochenska

Both the inputs provided by Elena V Romanova and Julius Pavlov are good. Its always better to have the same parameters across instruments for analysis. It always better to run standard and fine tune before runs.

Thank you.

Varsha