We are planning to do whole genome sequencing of a plant and have to send our samples to a respective foreign company to do that. In that case, we afraid to send the extracted DNA sample due to degradation possibility. Therefore, we may send the leaf sample instead of DNA sample. Our leaf samples are stored at -20 degree. My query is-
1. Is that okay to preserve leaf sample for genome sequencing at -20 degree? If not, what will be the appropriate procedure to collect/ preserve leaf sample?
2. What is the procedure to transport leaf sample for genome sequencing from one country to another and what conditions should be followed?
3. Which one is better for transportation - Leaf sample or DNA samples?
Looking for expert opinions.
Would a vacuum sealing of the specimen help in this sample before freezing ? Just interested...Thanks .
Leaf sample is much better as i suggest because from several research studies i took part by collecting leaf samples in the field work stored in 20 dreg and after freeze dry and export to the lab for sequence them to get the result
It's better to transport DNA samples than Leaf samples. Anyway, it's my opinion!
What to do is place your DNA in a 1.5 or 2 mL screw cap microcentrifuge tube and seal with Parafilm. Pack the tube in a freezer box, 50 mL conical vial or some other method to protect it from breaking. Place into a thermo-stable shipping box. Styrofoam be at least 1.5 inches thick~~ recommend.
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