In regular macronutrients if we add urea as a supplementary nitrogen source, does it show regeneration in cotton embryos from those who failed to regenerate?
Genetic response of a species is important for regeneration. May be this factor affected regeneration in your work. However, if you want to increase the regeneration of a species you may check in different composition or amount supplementary N sources (urea or amonium nitrate) in regeneration media. Regads. Cigdem.