For example, Roche has discontinued its Universal Probe Library Products and the Assay Design Centre (https://lifescience.roche.com/global_en/brands/universal-probe-library.html#assay-design-center).
Changing the manufacturer can potentially change the previous results done using those products. Researchers whose previous researches were conducted based on those products will have to validate all those results again using new products from a different manufacturer to continue their researches. This can waste lots of fundings.
What are your opinions regarding this problem?
Not exactly. Although it may have some impact on academic achievement like citation score. It also depends on many thing like nature of product and most importantly structure of market.
No, simply we can say those research will give some fundamental knowledge regarding the penetration of a new product also.
No
it helps inform future research on substitute products and supports research and development of new products
If anyone wants to keep using their Roche UPL probes after this service is discontinued, we created a replacement web app that anyone can use for free here:
https://primers.neoformit.com
Great job Cameron
do u have also all the sequences of the probes as well?
Cameron Hyde , thank you so much for sharing all this info.
We want to continue using the UPL system in our lab and I'm just wondering about the reference that they "contain" LNA. Any idea if it is all of the 8 nucleotides? I'm thinking ordering a DNA only probe will have an impact on hybridisation efficiency/specificity.
Thank you all for this great thread.
Product discontinuation as in this case is a serious problem and IMHO the company should just share the product info freely so the community can keep working as before.
in the patent
EP2495336A1 - A new type of universal probes for the detection of genomic variants - Google Patents
is written that should have a structure as:
probes having eight nucleotides with the general structure 5'-D - L - L - L - L - L - X - X - 3' or 5'-D - L - L - L - L - X - X - X-3', wherein D is a DNA nucleotide, each L is a LNA nucleotide and each X is a LNA random nucleotide
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