Please suggest me the simplest way to understand the in-silico analysis of any gene?

I want to understand the basics of docking, and also know about all bioinformatics tools which are free of coast and helpfull in in-silico analysis?

22 February 2021 8,879 2 View

Is the assumption about Delaunay edge true?

01 July 2020 9,998 2 View

What will be the roadmap to implement in matlab the power adaptation at transmitter side for optical wireless communication?

In my research, I have to implement a power adaptive transmitter in free space optical communication. Please guide me.

27 April 2020 2,406 2 View

How magnetic nanoparticles assembled with polymer and molded to form hard mesh for water purification?

Starting source for Magnetic nanoparticles synthesis? Best polymer? Efficient Plant extract? Development of filters for water treatment References? Thanks in advance!

19 August 2018 2,193 3 View

What percent the co-culture of cellulose fermentation with fungus and ethanol production with yeast is successful?

I am trying to set up co-culture study experiments for cellulose fermentation by fungus and subsequently ethanol production with yeast. The product of cellulose degradation in first step is...

06 August 2018 2,896 3 View

How can I determined average size of nanoparticles from TEM data?

We synthesize silver nanoparticles, using biological methods, in TEM image there are variety of SNPs size like, 13 nm, 16.15 nm, 20 nm, 16.39 nm.

31 May 2018 9,409 3 View

Synthesis of nanostructure material like silver and gold using protein molecule, has more stability, what is the possible phenomenon?

Protein play vital role in synthesis of various nanomaterials, synthesize nanomaterials having more stability and unique SPR peak as compare other methods. Cysteine amino acid may involve for...

30 May 2018 3,662 3 View

While enzyme immobilized with nanostructure does optimum experimental condition is affected for enzyme?

We are doing enzyme kinetics with enzyme immobilized on nanostructure, we found that the enzyme activity is more efficient than its optimum experimental condition.

27 May 2018 480 5 View

What should be the ratio of silica ( 60 - 120 mesh size) and plant extract in column chromatography ?

12 August 2017 8,193 3 View

Temporal associations of dengue fever and NDVI, LST and TRMM (Precipitation)?

Looking for the literature on temporal association of dengue and satellite derived variables (NDVI,LST, Precipitation etc)

06 June 2017 679 6 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View