Excitation wavelengths must be 280 nm or 295?
hello everyone, I have been trying to overexpress MDR1 gene using lentiviral vector transduction in MDA-MB231 cancer cell line. The cells were successfully transduced with virus (MOI: 10) and...
09 October 2017 1,033 6 View
I want to transduce MDA-MB-231 cells with lentivirus in order to achieve a stable expression of MDR1. But unfortunately, after transfection and antibiotic selection, the cells die after they are...
07 August 2017 1,039 6 View
unable to create log file in temporary directory:/home/ligplus/lib/params/ligplus.par i download the components.cif. its need to change the ligplus.par file in the params folder??? Can anyone help...
07 August 2017 4,497 2 View
For difference wavelength 60nm (Trp) with excittion 280nm we have seen max emission in 340nm. If the distance between the excitation wavelength (280) and emission wavelength (305) is about 25 nm,...
31 December 2014 1,023 3 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
