Sadegh Farhadian

5 Questions 7 Answers 0 Followers

Questions related from Sadegh Farhadian

How can I overexpress gene of interest using lentiviral system?

hello everyone, I have been trying to overexpress MDR1 gene using lentiviral vector transduction in MDA-MB231 cancer cell line. The cells were successfully transduced with virus (MOI: 10) and...

10 October 2017 1,011 6 View

Can anyone tell me how to run the LigPlus in UBUNTU (I have tried, but I failed to follow the LigPlus manoual v1.4)?

unable to create log file in temporary directory:/home/ligplus/lib/params/ligplus.par i download the components.cif. its need to change the ligplus.par file in the params folder??? Can anyone help...

08 August 2017 4,476 2 View

Has anyone ever tranduced MDA-MB-231 with lentivirus?

I want to transduce MDA-MB-231 cells with lentivirus in order to achieve a stable expression of MDR1. But unfortunately, after transfection and antibiotic selection, the cells die after they are...

08 August 2017 1,016 6 View

Is someone working with synchronous fluorescence?

For difference wavelength 60nm (Trp) with excittion 280nm we have seen max emission in 340nm. If the distance between the excitation wavelength (280) and emission wavelength (305) is about 25 nm,...

01 January 2015 1,002 3 View

If I want to study synchronous fluorescence of tryptophan, what emission wavelengths and excitation wavelengths should I use?

Excitation wavelengths must be 280 nm or 295?

01 January 2015 9,324 0 View