If you plant an in vitro hairy root culture, it is unlikely to grow into a whole plant or develop shoots. Hairy root cultures are typically established by infecting plant tissues with Agrobacterium rhizogenes, a bacterium that induces the formation of hairy roots. These hairy roots are genetically transformed and can exhibit rapid growth and production of secondary metabolites.

In the absence of shoot induction or whole plant development, the hairy roots will primarily continue to grow underground and establish a root system. The roots may continue to produce secondary metabolites or other desired compounds depending on the purpose of your culture. It is important to provide appropriate growth conditions, including a suitable growth medium, nutrient supply, and proper aeration for the roots to grow and thrive.

Keep in mind that the growth and behavior of hairy roots can vary depending on the specific plant species, the type of transformation, and the culture conditions. Therefore, it is always recommended to consult scientific literature or seek guidance from experts with experience in working with in vitro hairy root cultures for your specific plant species or research objectives.

Thank you Srikanta,

Chris