Buffer for protein solubilization after acetone/AS precipitation that is compatible with cell stimulation?

Hello! I am precipitating some proteins (with either acetone or ammonium sulphate) and I need to resuspend the protein pellet in a buffer that properly keeps the proteins soluble AND which is...

27 January 2021 6,958 4 View

Is there an efficient way to sample moths without the need to check the traps every day?

I would like to sample moths during the night in a remote area, so I cannot go there as frequently as I would like to. I would be happy to track changes in moth abundance from week to week. Is...

26 November 2018 576 17 View

How to genotype the arabidopsis mutant eds5-1? How to find the exact place of the SNP?

How to genotype eds 5-1 arabidopsis mutant? It's SNP but no idea where it is. Articles were found only with contradictory primer sets. Every articles are citing the same author and journal...

25 June 2018 4,559 3 View

Best way for His-tag purification?

Hello, I would go for His-tag purification and I was wondering which company would be the best to order Ni-TA column? Do you have a preferred one or all of them would be the same? Thanks in...

03 April 2017 6,420 4 View

Sf9 cells won't transfect... Any suggestions on how to improve?

I am working with the bac-to-bac expression system and following the Invitrogen protocol available online. I have generated my recombinant bacmid and now I should transfect my cells, though they...

16 May 2016 4,906 2 View

Does somebody know/have a meta-analysis of public health campaigns efficacy aiming obesity?

Is there an evaluation on how most of the campaigns are designed?

30 March 2016 903 4 View

Would a paper-made psychometric application remains publishable for a I.F. journal?

Nowadays is clearly the advantages of electronic tools (as programs and computers) for cognitive studies and its prevalence in current publications (specially for the neuroscience field). Then,...

16 August 2015 9,212 3 View

Are NK92MI cells smaller when they are in low cell number?

I am trying to culture NK92MI cells, but they seem to be extremely small. I tried to subculture them in T25 flask (as described by ATCC ~200 000 cell/ml, in the complete growth medium they...

12 March 2015 2,218 2 View

Does anyone have an idea what can be the problem during our RNA ISH on free-floating mouse brain sections?

22 February 2015 4,855 7 View

Puromycin resistant 293T stable cell line - can anyone help?

I am trying to make a stable cell line that expresses my protein of interest. I have the gene of my protein in a vector that provides puromycin resistant, but I am not sure what protocol to use....

08 September 2013 9,343 5 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to calculate battery consumption for IoT device?

Hi, everyone I'm working on a privacy-preserving algorithm that will apply to IoT devices. So, I need to measure the power consumption by implementing this algorithm. Any suggestion, advice,...

25 February 2021 8,749 6 View

How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT?

Im looking for any papers, reports,articles, links etc on information on How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT.

24 February 2021 2,750 4 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

How to construct different filler shape in MD simulations?

Is there any way to construct different initial filler shapes like triangle, square, rectangle, pillar, and tube in any simulation software.

20 February 2021 1,750 1 View

I am looking for a IoT simulator ?

I am working on a university project so, I m looking for a good Simulator compatible with LoRa and LoRaWAN protocol to simulate an IoT scenario. Thanks in Advance

20 February 2021 6,352 6 View