I have to analyse a compound by ESI-MS. if it is not soluble in Methanol, ACN and water. Then what can i do? my compound is chloroform soluble.
tripti can you tried different ration with methanol and water. pl try and get back me
i have tried in 50:50 Methanol:Water. but it is not soluble in so. DART-MS can be helpful but instrument is not working.
Try the different ratio of solvent and heating with sonication... It could dissolve at least the amount needed to be analysed by ESI-MS.
First of all thank you sir for your suggestions. I will try and get back to you.
look up the properties of the compound see what solvents they're soluble in, it's possible they may be difficult to analysis by lc ms ms, gc ms ms may be your only choice.
Your compound sounds very lipophilic. Are you using a C18 or C8 HPLC column or are you direct injecting the material without a column? If the material is very lipophilic, it may not elute from C18 or C8 without tetrahydrofuan (THF) in your organic mobile phase (for lipids 20-40% THF in ACN is used for lipid analyses).
If your compound is that non-polar, you may be able to dissolve in THF and dilute slightly with water (50% max) before you analysis. If your compound elutes early on these columns, then I would try isopropanol instead of THF.
Tripti Mishra,
ESI-Ms is a very sensitive technique and the least unimaginable soluble amount will be detected.
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