Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Software for flow cytometry-like gating of data sets?

I am currently trying to do image based cytometry from fluorescence microscopy images. From the images I extract data such as cell size and a number of fluorescence intensities. Is there any...

09 February 2021 6,278 1 View

Can A. thaliana splice algae genes?

24 January 2021 329 2 View

Does long term preservation (10years) of Collembola in 99% Ethanol affect recovery rate of extraction by flotation?

Hello! I am currently developing an automated counting approach using Fiji to count Collembola from pitfall trapped samples that have been stored in Ethanol (99%) since 2010. I use flotation (tap...

13 January 2021 659 2 View

Co-stain Lectin PNA FITC conjugate with an uncojugated primary antibody?

Hello, I am planning to stain retinal tissue sections for IHC and want to co-stain cones and rods. For this we plan on using a Lectin PNA From Arachis hypogaea (peanut), FITC Conjugate for cones...

12 January 2021 583 1 View

How to use Abaqus USDFLD subroutine to vary stiffness?

Hi I have a problem where I am trying to vary the stiffness spatially across a structured interface. The whole idea is to vary the stiffness vertically, with a bulk stiffness of 8GPa decreasing...

06 January 2021 262 3 View

I really need help with cov-2 pseudotyped lentivirus production. Support?

Hi, i'm desesperate. i'm trying to produce lentivirus usinf BEI resources kit NR-52948. 24 hours after transfection i detect a massive GFP expression in my 293T cells. After 72 hours i filtered...

30 December 2020 9,889 6 View

How can i estimate the total phenolic content from a plant extract?

Suggestions and recommendations are welcome. A protocol used and experience would also be helpful.

25 December 2020 9,807 4 View

I need to accelerate corrosion in steel parts that are used in a salt-water environment. Is there a technique for doing so?

I have a structure that employs d-ring swiveling tie-downs that corrode during use and become "frozen". I have sample swivel assemblies that I wish to corrode to see if I can free them using one...

01 March 2021 3,739 3 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

I accidentally poked my finger with very thiny tweezers after I used them to remove a slide from A549 cell culture??

I accidentally poked my finger with very thiny tweezers after I used them to remove a slide from A549 cell culture. I got poked after sprayed them with 70 % alcohol and dried them right away. Is...

07 January 2021 9,412 2 View

How Can conduct measurement on powder using Raman Microscopy?

I am trying to measure the Raman microscopy spectrum of a drug in its powder form and I am not sure what is the best way to spread the powder over the microscope slide so that no interference will...

13 December 2020 7,884 6 View

I need Coronary Angiogram Images for Coronary Artery Disease detection?

I need Coronary Angiogram Images(CAI) publically available dataset. Coronary Artery Disease (CAD) is a condition of the heart due to atherosclerosis. Atherosclerosis is the narrowing of arteries...

07 December 2020 6,470 3 View

I messed up the DMSO and serial dilution standards in my MSD assay...How can I save my data?

MSD requires 8 standards that are serially diluted down in order to produce a standard curve. In the final row of my MSD Assay, I accidentally pipetted DMSO (same volume) into the last 4 wells of...

24 November 2020 3,376 2 View

What could be the best genetic markers for cuticle resistance in Mosquitoes ?

Insect cuticle plays control roles in reducing insecticide penetration into the insect's internal body. This cuticle possesses cuticular biomolecules such as proteins and hydrocarbons which maybe...

11 November 2020 6,333 1 View

I am looking for advice. How to prepare bacterial cells for AFM images (live and fixed on glass slide)?

We have a new AFM with a capability to achive high resolution images (up to 50nm). The AFM tips can scan the surface of the object and as well, can penetrate into the object. We also have the...

07 November 2020 2,813 7 View

What is the best machine learning algorithm for construction accident data analysis?

Hello everyone! I am actually looking for experts opinion regarding which machine learning algorithm is best suited for construction accident data. Looking forward to getting your wonderful...

04 November 2020 1,046 9 View