17 June 2016 1 5K Report

Can anyone send me references or any information ?

More Ram S Puranam's questions See All
Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is it fare for editors of reputed Journals to put on hold manuscripts more than six months without review updates, Are they harassing researchers?

It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...

24 July 2024 8,596 2 View

Can I Use D-PBS for Preparing a 10% Sucrose Solution for Lentivirus Concentration?

Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...

21 July 2024 4,676 4 View

Unification of General Relativity and Quantum Mechanics is possible ?

How can anyone develop a theoretical framework that successfully unifies general relativity and quantum mechanics? how this new theory should accurately describe gravitational interactions at...

10 July 2024 5,040 8 View

The field of cosmology and general relativity is vast and intricate,mistakes in tensor calculations, which are fundamental in general relativity ?

The field of cosmology and general relativity is vast and intricate, with numerous potential areas for misconceptions and errors. Initial mistakes in tensor calculations are some common mistakes ?

27 June 2024 6,329 2 View

Sulphur test method for edible oil?

Hi everyone do you have any method for sulphur test in edible oil? as per lower cost, which instrument we can analyze sulphur in edible oil?

29 April 2024 9,789 0 View

How to write a research paper?

please tell the research paper writing steps from beginning to end.

24 April 2024 978 5 View

All the research papers (4-5) of PHD are in one journal, what impact will it have for future job in academic career?

If after doing PhD, a research scholar has 4 to 5 papers published, and all are published in the same journal (SCIE), then what impact does it have on his future teaching, academic career, or...

30 March 2024 3,615 1 View

Can anyone help me in interpretation the mass spectra ?

Dear Dr. thanks in advance. EI+ mass peak m/z = 55, 74, 84 (bp), 115, 129, 149, 191. is the above fragments possible from the compound smiles CC(OC1C=CC(=O)OC1C)C1CCC(=O)O1

21 March 2024 8,334 0 View

How can i need convert 1 ppb to Molar concentration?

Some researchers represents the solution concentration in Molars like the mM, uM, nM etc. and others represents in ppb or ppm. How can i need convert 1 ppb to Molar concentration?

24 February 2024 4,224 2 View

Similar questions and discussions
Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How can I get old refernce in geomorphological mapping?

how can I read Waters, R. S. 1958. Morphological mapping. Geography 43 :10-17 from internet? note: not in google or resaearch gate

26 July 2024 7,813 3 View

How to merge two or more linkage maps?

I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.

25 July 2024 6,505 2 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close