Can you suggest me a way to induce the sporulation of Alternaria tomatophila ?
Is their any relation with the age of conides and their potential to germinate ?
Most Alternaria species will sporulate on nutrient limited media, such as potato carrot agar or synthetic nutrient agar and exposure to cool white light. 30 min of direct sun light per day can also enhance sporulation. V-8 juice agar can also be used, but V-8 is not always easy to find.
In general the older the conidia, the less viable they will be, but it will greatly depend on the storage conditions (temperature, moisture, etc.). Alternaria conidia are relative hardy and can remain viable for some time.
Oh yes as Mariette claims above, use PCA potato carrot shoudl be enough for Alt solani complex incl your tomatophila. to enhance sporulation, always use light/dark alteration (no matter ambient or arteficial, UV is not recommendet can change the physio) simply after 3-4 day young colony cut off a pc ca 1x 3 cm, place it a side, then after some more days the new conidiophors should be up and bringing conida, just at the edges of the cutt off colony (cut colony it also with agar till the bottom), check it with low magnif 50-100 x microsc, directly through the plate. good luck and try to find all possible literature by Dr. Emory G. Simmon, his work (mainly in Mycotaxon and his rather recent book Alternaria, An identification manual,2007 CBS) will teach you everything about the traditional alternaria mycology. good luck! :-) as a student in alternariology you simply need it!
on the econd question, totally agreed as said/noticed above..
Dear Mariette ,
Actually , i am using PDA (potato dextrose agar ) and all my fungi ( such as A.solani , A.grandis , on both tomato and potato cultures ) , produce conidia , except one fungi : A.tomatophila on tomato , i have noticed that it was hard to get some spores from this fungi , but A.tomatophila , which was isolated from potato , can produce spores after 10 days of incubation
Regards,
Narimene.
Ps : i am testing the effect of sun light , i will check tomorrow if there is some conidia
Dear Roman ,
I have the first one : Mycotaxon , but the second , unfortunately , we dont have in our library.
If you have the electronic version , can you send it to me ?
Regards
NAarimene
Dear Oadi ,
when i have exposed my fungi to a photoperiod , i have noticed a production of differents pigmentations , and those fungi produced a lot of conidia , except A.tomatophila on tomato
For the UV , I agree with Roman
Regards
Send your institutional adress, I will help you by sending you a technical book.
Dear Mrs Narimene
this is your institutional adress : Avenue Hassan Badi - El Harrach - Alger 16040
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