I'm currently conducting research about the Efficacy of Neem Leaves and Calamansi Leaves Extract to Kill Golden Apple Snail (GAS). I'm currently stuck on my methodology because I don't know what is the proper concentration I should do. My adviser told me to conduct an LC50 to know which one is the best among them. He also recommended adding a commercial-based biopesticide to treatments. Overall, my treatment should be as follows:
(Total of 1L solution)
Control Group- 100% Water
Treatment 1- Neem Leaves Extract
Treatment 2- Calamansi Leaves Extract
Treatment 3- Commercial Based
My question is now what is the concentration for my treatment 1 to 3? If you have any suggestions please do reply and give me a reference as well because I need to cite them. Thank you.
Neem (Azadirachta indica) is recognized as a medicinal plant well known for its antibacterial, antimalarial, antiviral, and antifungal properties. Neem (Azadirachta indica) plants parts shows antimicrobial role through inhibitory effect on microbial growth/potentiality of cell wall breakdown. Azadirachtin, a complex tetranortriterpenoid limonoid present in seeds, is the key constituent responsible for both antifeedant and toxic effects in insects.
Aqueous neem leaf and seed extracts, neem oil and Bioblitz (EC formulation) against golden apple snail in the laboratory. Leaf and seed extracts are the most toxic causing 100% snail mortality at 100 ppm after 48 hours. Aqueous neem seed and leaves extract is tested against golden apple snails in the field . Treatment with concentration 20, 90 and 100 kg/ha of neem seeds are the most effective aqueous extract which inhibited feeding of the golden apple snails.
The toxicity of leaf crude extracts from neem tree and garlic (Allium sativum L.) on mortality rate of golden apple snails at concentrations of 50, 250, 500, 750 and 1000 mg/l. High concentration of neem tree leaf extract (1000 mg/l) killed 95.83% of golden apple snail in 95 hours and high concentration of garlic (1000 mg/l) killed 91.66% of golden apple snail in 96 hours.
https://www.intechopen.com/chapters/37961
https://www.researchgate.net/publication/8689026_Molluscicidal_effects_of_neem_Azadirachta_indica_extracts_on_edible_tropical_land_snails
https://www.google.com/url?sa=i&url=https%3A%2F%2Fbackyardgardengeek.com%2Fdoes-neem-oil-kill-snails-or-slugs%2F&psig=AOvVaw2KYShTQFY-J0NOxiVHqCCp&ust=1654500227881000&source=images&cd=vfe&ved=0CAwQjRxqFwoTCNDCiufjlfgCFQAAAAAdAAAAABAD
https://www.google.com/url?sa=i&url=https%3A%2F%2Fdavesgarden.com%2Fguides%2Farticles%2Fusing-neem-oil&psig=AOvVaw2KYShTQFY-J0NOxiVHqCCp&ust=1654500227881000&source=images&cd=vfe&ved=0CAwQjRxqFwoTCNDCiufjlfgCFQAAAAAdAAAAABAX
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