I would like to take bacterial OD600 measurements on a microplate reader? How can I do that?

06 June 2019 1 7K Report

I understand the OD measurement in a plate reader varies with the volume of the sample measured. Is there a formula to correct for path length without finding the correction factor by myself?

Engelbert Buxbaum

OD600 is a semi-quantitative measure of turbidity and hence bacterial density. Although the Lambert-Beer law doesn't apply to light scattering, the OD600 is a reasonable linear function of bacterial density, if always measured under the same conditions. So just fill all wells with the same volume. You can make a standard curve (OD600 vs bacteria/ml), but that is rarely worth the trouble.

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