Calculate the fluorescein concentration from the calibration curve. Substrate the control reading (hydrolysis in absence of soil) from the sample reading (hydrolysis in presence of soil) and express activity as " micro-gram of released fluorescein per gram soil dry mass over hour" using the following formula:

Micro-gram Fluorescein/ (g dry soil x h) = Sample - Control / Ws x Ds

Sample = pNP concentration of the soil sample (micro-gram)

Control = pNP concentration of the Control ( micro-gram)

Ws = Wet soil weight (g)

Ds = Dry soil weight (g)

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I have a treatment contain enriched compost with microbes. I need to evaluate the microbes but don't want to proceed a CFU. Can I use the t60 uv vis spectrophotometer ?. And if yes what procedure to follow