Which anesthetic for cortisol determination in fish?

I am a bit confused. I am planning an experiment in the field where fish will be caught by electrofishing. Afterwards, they are supposed to be euthanised by using an anaesthetic. I found...

10 February 2021 1,101 4 View

How to remove BF3 etherate from a reaction mixture?

20 January 2021 6,322 2 View

Why my BOC-protected compounds got deprotected during evaporation?

I purified my Boc-protected molecule using HPLC. The eluent is ACN/H2O/(0.05% TFA) . After I evaporated the solvent (65 oC), the product was collect as the MS result showed ~20% of the product was...

14 January 2021 5,487 5 View

Can anyone help me with the protocol of intranasal administration to mice ?

i wanna administer cigarette smoke extract to induce emphysema in mice, i am asking for the maximum volume of intranasal administration ? can i use ether or chlorofom to anesthetize mice?

10 December 2020 6,319 4 View

What is the cause of abnormal breathing in anaesthetized rats?

Is anyone able to tell me why my anaesthetized rats often do a 'double breath', or diaphragmatic breath, like a hiccup (see video). This often begins after 1-2 hours under anesthesia, and can...

06 December 2020 2,514 5 View

Should I use anesthetics before guillotine If I inteed to see brain inflammation?

Hello, I also want to do some biochemical analyses and need to take some blood of the rats. To take the blood I need to use anesthetics. But I want to evaluate TNF-a and oxidative stress in the...

19 October 2020 3,835 3 View

Problem about rat anesthesia?

I have a problem about rat anesthesia, the situation is that I have Zoletil 50 1mg/ml + xylazine hydrochloride 23.32mg/ml solution that I prepare at the same time from the same source (from the...

07 October 2020 9,140 7 View

Effect of anesthetics drugs on coronavirus patients?

What is the reason for the ineffective or insignificant effect of Neuromuscular relaxants such as Atracurium and anesthetics such as Ketamine on patients with Coronavirus hospitalized in the...

22 September 2020 5,734 4 View

Do you think we have found the Holy Grail of astrobiology? Are we not alone anymore?

Do you think we have found the Holy Grail of astrobiology and finally come close to proving that we are not alone in the universe? "Jane Greaves from Cardiff University in the U.K. and several...

13 September 2020 3,835 17 View

How to evaporate diethyl ether from mouse plasma samples?

What is the best way to evaporate diethyl ether from a mouse plasma extaraction? I am trying to extract indole from the mouse serum, using diethyl ether, and my sample size is very small (< 1...

28 October 2019 6,288 1 View