I am not sure about hemolymph specifically but do keep in mind that the anthrone assay is far from specific to glucose. Indeed it will readily react with many other sugars. See Cerning-Beroard (?) "A note on sugar determination by the anthrone method" available here:
If you want specifically glucose I would suggest using a glucose-oxidase kit or similar.
As Yoram Gerchman mentioned above there are some commercially available kits for glucose that are readily available. I've used them as well as the anthrone method in the past for measuring glucose in Callinectes sapidus.
Validating the use of portable glucometers (for diabetics) might be an interesting thing to do for field work.
A nice idea indeed! Do make sure the range is right. As those machines are designed to human blood they tend to give "High" and "Low" if you cross the borders. See bellow for discussion and keep in mind the limits might be different for different brands.
http://www.diabetesmine.com/2013/04/what-your-glucose-meter-may-know-but-isnt-sharing.html
Many thanks to you all, I harvested a lot. I will try to use a Glucose (GO) Assay Kit as seen in many reports. The anthrone test may be imperfect.
Keep in mind that glucose should be measured very soon after collection. Measurements from tissues frozen for a while may show marked declines.
Jeffrey D Shields
Thanks very much! I'll take care of it. And how about other tissue in the crab? How to collect, pretreat them for glucose measure? such as the hepatopancreas and stomach? Can you give me some advice?
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