How long and what percentage of PFA should we use to fix postmortem human brain sample in a cassette for IF staining?

I recently received postmortem (aged) human brain samples in a cassette (previously fresh frozen) from brain bank. I would like to use those samples for fluorescent immunostaining. Could anyone...

30 September 2020 1,914 2 View

What is the best method to isolate RNA from preserved blood sample which was in liquid nitrogen tank for a long time?

I have some patients cryopreserved lymphocytes samples which I want to analyze with RT-PCR later. It has been stored in the liquid nitrogen tank more than 2 years. I also want to use them for...

16 April 2019 425 4 View

Are there any reliable antibodies for excitatory (glutamatergic) and inhibitory (GABAnergic) neurons in hippocampus?

I have Camk2a-Cre mice model, and would like to have antibodies for excitatory (glutamatergic) and inhibitory (GABAnergic) neurons in hippocampus CA1 regions. Do you know any good antibodies for...

07 November 2018 5,076 3 View

How do you measure COMT enzyme activity? Any assay kit?

Does anyone know how to measure COMT (cathecol-o-methyltransferase) enzyme activity? Thank you.

14 August 2018 9,026 2 View

Any (hippocampal) culture and cell line useful to investigate epigenetic regulation (ie. high proliferation rate)?

I am currently looking for (preferably hippocampus) culture/neurosphere/cell line with high proliferation rate, which are available to purchase in Canada.

12 February 2018 3,022 2 View

Do you have any suggestions for neurosphere assay protocol, using adult mice?

Do you have any suggestions for growth factors (EGF and FGF) effective/useful for proliferation medium? How do you sterilize surgical equipments - autoclave or buffer? Any tips to share -...

12 February 2018 2,266 2 View

How can I prove some claim?

I saw a claim in some paper without proof. Because the formula is complicated, I wrote it in the attached file. I can not prove one part of the assertion. Please tell me how to prove it if you can...

31 August 2017 7,223 7 View

Is an integrand convergence necessary for the improper integral convergence?

01 February 2017 5,198 5 View

Is Lyapunov's second method useful for linear system?

20 April 2016 4,301 14 View

What is a definition of a limit point of a function?

29 March 2016 5,013 9 View

How to compute the internal consistency of a scale with ordinal items?

Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...

28 February 2021 1,369 5 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

Latent profile analysis based on two variables/sources of data?

Hello, for my master project, I would like to conduct a latent profile analysis about self-regulated learning skills. I would like to take two different measures into account when creating the...

21 February 2021 2,326 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

How to calculate the sample size in an interobserver variability study?

Hi I am conducting an interobserver variability study where we have 12 raters who are going to rate samples of lesions. They will rate up to 10 variables per sample. Although the size of the...

12 February 2021 7,276 3 View