- Chickpea seeds germinate at an optimum temperature (28-33°C). I will grow plants at 28/16°C in a growth chamber and then expose them to high temperatures at the first appearance of flowers.
- I want to know that for how long this heat treatment should be given and for how many days? Let's say Should the temperature in the growth room be increased daily by 1°C, e.g. 28 to 40°C during the day and 16 to 25°C during night. or should I give the heat treatment of 35°- 40°C for 5 hrs everyday in a separate chamber and then place them back to growth chamber having 28/16°C temperature.?
Kindly refere to Devasirvatham et al. 2012, 2013. She did some of similar kind of work.
You need decide whether you want to see the effect of increased day temperature/ increased night temperature or combination of this. You haven't mentioned the purpose of the study in the question. Just to expose plants for short time and collect samples for molecular analysis/ you want to expose them to heat treatment and get the yield after the plants were exposed to heat stress.
In my view, chickpea plants often experience heat stress due to combined increased high temperature and low RH during reproductive stage. So care should be taken not only with temperature settings but also with relative humidity settings. If you want to take both the parameters then you can simply calculate Vapor pressure deficit (VPD) and maintain high VPD condition in growth chambers.
Thanks ma'am. I have gone through the paper you referred. They have increased the temperature daily from 28°C to 40°C to see the effect on reproductive development of chickpea.
But in some papers :-
Proline induces heat tolerance in chickpea (Cicer arietinum L.) plants by protecting vital enzymes of carbon and antioxidative metabolism
Neeru Kaushal, Kriti Gupta, Kalpna Bhandhari, Sanjeev Kumar, Prince Thakur, Harsh Nayyar
Heat stress is given in chickpea by directly placing the plants from 25°C to 35°C.
I want to give heat stress for screening some genotypes of chickpea plants. I just want to know whether I should increase the temperature gradually for giving heat stress or I should directly give heat stress at high temperature.
Hallo Ms. Pareek,
I must agree with the comment made by Ms. R. Pushpavalli . You need to decide the purpose for your treatment.
If you want to screen for high temperature tolerance plants, I would suggest to increase the temperature gradually in the Controlled Environment Rooms. I am not familiar with chickpea. However, in Brassica, the arrangement of humidity and water maintenance are very important during heat treatment.
Thanks Ma,am.
Purpose of treatment is to screen some genotypes of chickpea plants. I will follow the protocol keeping in mind all the conditions recommended by you and Ms. R. Pushpavalli.
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