I want to design primers for real-time PCR for the Homo sapiens paxillin (PXN) gene, which has several variant transcripts, using Allele ID software?

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How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

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Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

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06 August 2024 9,986 2 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

How are Large Models Exploring and Outputting Knowledge Understanding in Specific Content Areas, and What Does Academic Research Say About It?

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05 August 2024 5,729 2 View

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How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

What is the best sampling strategy?

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01 August 2024 8,457 10 View

What is the problem with these tissue culture plants?

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01 August 2024 589 4 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

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01 August 2024 9,227 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

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08 August 2024 4,645 2 View

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Is this website real?

Is this website real? https://isar.org.in/event/registration.php?id=2434532

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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Why does the MFDFA algorithm need to calculate the profile of the time series?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Is there any machine to do real time pcr?

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Katie A S Burnette

Do you want advice about the software or about qPCR primer design or both? I can help with qPCR design considerations, but not the software.

Frederic Lepretre

Hi Ali

by the UCSC genome browser you can access to the gene sequence and with its optional tracks view the exiting primers. I just advice you to take the ones at most of the 3'end of the gene, eg at left in the picture, and encompassing all transcripts.

best

fred