I want to ask how do i select HKY MODEL IN MEGA?

06 December 2023 0 8K Report

Can some one please tell me what can i do? or do I select some other model?

Badges
Science topic

Are international conferences a dying species?

In our discipline (chemistry), annual meetings of the American Chemical Society (ACS) used to be mega-events with up to 20,000 participants. The current one will be virtual, and the organizers...

06 January 2021 1,882 5 View

Why are the ClustalW (codon) and MUSCLE (codon) alignment options greyed out in MEGA-X?

Hi, I'm currently trying to do a multiple sequence alignment for a gene family using the coding nucleotide sequences. I want to create an alignment based on the amino acid sequences but that is...

28 July 2020 2,709 3 View

How many references sequences should use for a phylogenetic tree construction?

I have identified fungus isolates from the fruit surface by SSU +ITS+ LSU regions, such as : 1) Fusarium kyushuense , 2) Fusarium proliferatum ;3) Aspergillus aculeatus ;4) Aspergillus europaeus...

15 July 2020 5,573 2 View

Why is a DNA partial sequence (16s rRNA) placed in a separate branch in phylogenetic tree?

Dear all, I have some bacteria DNA partial sequences (16s rRNA). They sequenced only by 9 F primer. Their length is under 1100 bp. When I draw phylogenetic trees for some of them by MEGA, my...

23 April 2020 9,203 8 View

How to align multiple sequences (different genes) to a reference/query sequence (complete genome)?

I am working on FIV and have sequenced each gene individually and retrieved multiple complete genomes and individual genes from Genbank. I am trying to align all the different genes to the...

02 March 2020 1,419 1 View

How to edit node labels in Phylogenetic tress?

I have constructed phylogenetic tree in Mega software (attaching snapshot), importing 'FASTA format' data from NCBI. According to editor, Nodes labelling should be in order: Genus, species,...

01 February 2020 2,074 4 View

how to export a phylogenetic tree into word document using MEGA X?

I have built a tree of my interest but MEGA X displays an error when saving the image as PDF file! I was wondering how can i save/ copy the tree into a word file. Thanks all

09 October 2019 4,045 5 View

How do you combine multiple SEQ files iinto one file (FASTA or notepad)?

I use MEGA X for my phylogenetic analysis and sometimes I may work with 70+ sequences. If any of you know a method that can combine these sequences into one and free me from the ////tedious////...

31 July 2019 2,969 3 View

What details should be followed in MEGA-X for the Multiple Sequence Alignment of 16S data?

I have some 16S RNA sequence with which I want to do the phylogenetic analysis, so Initially I did BLAST against SILVA db and NACBI 16S db and went for MSA in MEGA-X using Muscle and Clustal both...

21 July 2019 9,320 3 View

Analysis and Phylogenetic tree for a fasta file?

I have a *.fasta file through nanopore sequencing (Min Ion) of approx ~250MB. I'm having problem in its analysis via MEGA Software due to large file size. I need to select a particular bacterial...

05 June 2019 5,003 6 View