I try to answer the message from a researcher. But there is a message "something went wrong. try again later" in many times Why?

More Yoshiki Yamagiwa's questions See All

What is the meaning of WGT entry in SDEF card for MCNP?

Hello all. I'm using MCNP6 to get detector response with F8 tally. In some documents, it is mentioned to use WGT entry in SDEF card to scale source intensity and to get correct results for...

26 March 2019 8,649 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Do you agree that at some point in our lives each of us will be a person with disabilities?

There is several approaches towards the analysis of legal status of people with disabilities (e.g. medical, social or human rights approach). One way to include people with disabilities in society...

17 July 2024 3,798 13 View

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized?

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...

13 July 2024 2,431 2 View

Solvent recovery after extraction - Separation of Isopropanol + Water - how to do it best?

I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...

08 July 2024 9,201 2 View

Why liposome size increase during storage?

My liposome is a non-phospholipid liposome (sterosome) composed of palmitic acid and cholesterol, 20mg of palmitic acid and 70mg of cholesterol. After several months of storage in a refrigerator...

18 June 2024 7,459 5 View

How can i use hexamethyldisilazane(HMDS) for drying instead of Co2 and freez drying for SEM analysis of rice anther and pollen?

could you please help with a protocol? should i do it directly on the aluminium stub after dehydration with 100% alcohol or not? should i release the pollen or whole anther is better and dehisce...

03 June 2024 4,793 8 View

Can HCl be used instead of H2SO4 for RNA later preparation?

I am preparing RNA later following the patented protocol. It is mentioned there and in other recipes as well to use H2SO4 to balance the pH to 5.2. If H2SO4 is not available, can I use HCL instead...

30 May 2024 3,220 0 View

Cell culture contamination?

Hello everyone, Recently, I have encountered a problem in cell culture. I use Rpmi 1640. I have freezed SKBR-3 cells 8 months ago. I am defreezing them now. I defreezed two cryovials up to now and...

22 May 2024 8,278 4 View

How would one prove that a lateral shift is directly proportional to refractive index and what is the crystal with the highest refraction index?

18 May 2024 9,078 0 View

Adriana Guedes

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Nurul Ashikin Mohamed Shahrehan

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